The three most common for all is the Lowry protein assays (advanced copper), Smith (bicinchoninic acid, BCA) and Bradford (Coomassie Blue) method. All the colorimetric method, and based on production in the absorbing species with the proportion of the amount of protein in the sample. Ninhydrin assay which is a new method promises to be reported.
Lowry method
The Lowry, or increased alkaline copper method that starts with the addition of an alkaline solution of Cu2 + on the test. Copper is a complex with the nitrogen atom in peptide bonds of proteins under this condition, and reduced to Cu +. Read More
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