Ninhydrin-based assay method is based on the calculation of the total amino acids following acid hydrolysis of protein in the sample network. Microtitre plates used in this test. Tissue samples (10 mg) was first hydrolyzed in 500 ml 6 M HCl at 100 C for 24 h to liberate ammonia. Example and then lyophilized (cooled and evaporated), and the rest with ammonium chloride, used in a known volume of water.
Ninhydrin with ninhydrin reagent, ethylene glycol, acetate buffer, and tin-suspension, which was originally a bright red, is 1-10 mg protein hydrolyzate in flat-bottom microplate. During the 10-min incubation at 100 C, ammonia reacts with ninhydrin reagent to diketohydrin dylidenediketohydrindamine. Read More
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