Bradford method that has been shown to significan

Bradford Bradford method is based on the method of noncovalent binding of the anionic form of dye Coomassie Blue G-250 with the protein. Especially the dye reacts with arginine residues, one positively charged side chain, and light interaction is also observed with basic residues (histidine and lysine) and aromatic residues (tyrosine, tryptophan and phenylalanine). In the absence of protein, the dye-reagent is a red light, and the binding protein, a blue color with absorption maximum at 590 nm

Bradford exam that is very popular because of fast (5 minutes) and with one additional dye reagent to the sample. Offer non-linear calibration curve of the concentration of A590-20 in the 0.2-mg of total protein in the sample container volume of 20 ml (10-1000 mg / ml). Read More